作者
Yorick Post, Jens Puschhof, Joep Beumer, Harald M Kerkkamp, Merijn AG de Bakker, Julien Slagboom, Buys de Barbanson, Nienke R Wevers, Xandor M Spijkers, Thomas Olivier, Taline D Kazandjian, Stuart Ainsworth, Carmen Lopez Iglesias, Willine J van de Wetering, Maria C Heinz, Ravian L van Ineveld, Regina GDM van Kleef, Harry Begthel, Jeroen Korving, Yotam E Bar-Ephraim, Walter Getreuer, Anne C Rios, Remco HS Westerink, Hugo JG Snippert, Alexander van Oudenaarden, Peter J Peters, Freek J Vonk, Jeroen Kool, Michael K Richardson, Nicholas R Casewell, Hans Clevers
发表日期
2020/1/23
期刊
Cell
卷号
180
期号
2
页码范围
233-247. e21
出版商
Elsevier
简介
Wnt dependency and Lgr5 expression define multiple mammalian epithelial stem cell types. Under defined growth factor conditions, such adult stem cells (ASCs) grow as 3D organoids that recapitulate essential features of the pertinent epithelium. Here, we establish long-term expanding venom gland organoids from several snake species. The newly assembled transcriptome of the Cape coral snake reveals that organoids express high levels of toxin transcripts. Single-cell RNA sequencing of both organoids and primary tissue identifies distinct venom-expressing cell types as well as proliferative cells expressing homologs of known mammalian stem cell markers. A hard-wired regional heterogeneity in the expression of individual venom components is maintained in organoid cultures. Harvested venom peptides reflect crude venom composition and display biological activity. This study extends organoid technology …
学术搜索中的文章
Y Post, J Puschhof, J Beumer, HM Kerkkamp… - Cell, 2020