作者
Milou ML Dingemans, Aart De Groot, Regina GDM Van Kleef, Åke Bergman, Martin Van Den Berg, Henk PM Vijverberg, Remco HS Westerink
发表日期
2008/5
期刊
Environmental health perspectives
卷号
116
期号
5
页码范围
637-643
出版商
National Institute of Environmental Health Sciences
简介
Background
Oxidative metabolism, resulting in the formation of hydroxylated polybrominated diphenyl ether (PBDE) metabolites, may enhance the neurotoxic potential of brominated flame retardants.
Objective
Our objective was to investigate the effects of a hydroxylated metabolite of 2,2′,4,4′-tetra-bromodiphenyl ether (BDE-47; 6-OH-BDE-47) on changes in the intracellular Ca2+ concentration ([Ca2+]i) and vesicular catecholamine release in PC12 cells.
Methods
We measured vesicular catecholamine release and [Ca2+]i using amperometry and imaging of the fluorescent Ca2+-sensitive dye Fura-2, respectively.
Results
Acute exposure of PC12 cells to 6-OH-BDE-47 (5 μM) induced vesicular catecholamine release. Catecholamine release coincided with a transient increase in [Ca2+]i, which was observed shortly after the onset of exposure to 6-OH-BDE-47 (120 μM). An additional late increase in [Ca2+]i was often …
引用总数
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MML Dingemans, A De Groot, RGDM Van Kleef… - Environmental health perspectives, 2008