作者
Anna Charlotte Schultz, Sylvie Perelle, Simona Di Pasquale, Katarina Kovac, Dario De Medici, Patrick Fach, Helle Mølgaard Sommer, Jeffrey Hoorfar
发表日期
2011/3/1
期刊
International Journal of Food Microbiology
卷号
145
页码范围
S158-S166
出版商
Elsevier
简介
Enteric viruses, including norovirus (NoV) and hepatitis A virus (HAV), have emerged as a major cause of waterborne outbreaks worldwide. Due to their low infectious doses and low concentrations in water samples, an efficient and rapid virus concentration method is required for routine control. Three newly developed methods, A, B and C, for virus concentration in bottled water were compared against the reference method D: (A) Convective Interaction Media (CIM) monolithic chromatography; filtration of viruses followed by (B) direct lysis of viruses on membrane; (C) concentration of viruses by ultracentrifugation; and (D) concentration of viruses by ultrafiltration, for each methods' (A, B and C) efficacy to recover 10-fold dilutions of HAV and feline calicivirus (FCV) spiked in bottles of 1.5L of mineral water. Within the tested characteristics, all the new methods showed better performance than method D. Methods A, B …
引用总数
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学术搜索中的文章
AC Schultz, S Perelle, S Di Pasquale, K Kovac… - International Journal of Food Microbiology, 2011