作者
Shahriar Koochekpour, Michael Jeffers, Shen Rulong, Gregory Taylor, Eric Klineberg, Eric A Hudson, James H Resau, George F Vande Woude
发表日期
1997/12/1
期刊
Cancer research
卷号
57
期号
23
页码范围
5391-5398
出版商
The American Association for Cancer Research
简介
Using double immunofluorescence staining and quantitative confocal laser scan microscopy, we show that the intensity of hepatocyte growth factor/scatter factor (HGF/SF) and Met staining in human primary brain tumors increases with the grade of malignancy and is prevalent in both the infiltrating tumor cells and endothelial hyperplastic areas. HGF/SF and Met also are expressed in vitro in glioblastoma multiforme cell lines as well as in normal human astrocyte (NHA) cells. Moreover, HGF/SF stimulates tyrosine phosphorylation of Met in both glioma cell lines and NHA cells, but only the glioma cell lines proliferate and become motile and invasive in response to HGF/SF, whereas the NHA cells are nonresponsive. These results implicate autocrine/paracrine Met-HGF/SF signaling in glioma tumorigenesis and suggest that HGF/SF signaling through Met is negatively regulated in NHA cells.
引用总数
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S Koochekpour, M Jeffers, S Rulong, G Taylor… - Cancer research, 1997