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Although atopic dermatitis/AD often presents in infancy and persists into adulthood, comparative characterization of AD skin among different pediatric age-groups is lacking. This study aimed to define skin biopsy profiles of lesional and nonlesional AD across different age-groups (0-5 y/o infants with disease duration< 6 months, 6-11 y/o children, 12-17 y/o adolescents,≥ 18 y/o adults) versus age-appropriate controls. We performed gene expression analyses by RNA-sequencing and real-time polymerase chain reaction and protein expression analysis using immunohistochemistry. Our results found that Th2/Th22-skewing, including IL13, CCL17/TARC, IL22 and S100As characterized the common AD signature, with a global pathway-level enrichment across ages. Specific cytokines IL33, IL1RL1/IL33R and IL9, often associated with early atopic sensitization, showed greatest upregulations in infants. Th17 …