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Water shortage threatens agricultural sustainability in the Huang-Huai-Hai Plain of China. Thus, we investigated the effect of supplemental irrigation (SI) on the root growth, soil water variation, and grain yield of winter wheat in this region by measuring the moisture content in different soil layers. Prior to SI, the soil water content (SWC) at given soil depths was monitored to calculate amount of irritation water that can rehydrate the soil to target SWC. The SWC before SI was monitored to depths of 20, 40, and 60 cm in treatments of W20, W40, and W60, respectively. Rainfed treatment with no irrigation as the control (W0). The mean root weight density (RWD), triphenyl tetrazolium chloride reduction activity (TTC reduction activity), soluble protein (SP) concentrations as well as catalase (CAT), and superoxide dismutase (SOD) activities in W40 and W60 treatments were significantly higher than those in W20. The RWD in …