作者
R Preston Mason, Ruslan Kubant, Gehan Heeba, Robert F Jacob, Charles A Day, Yehudi S Medlin, Philipp Funovics, Tadeusz Malinski
发表日期
2008/8
期刊
Pharmaceutical research
卷号
25
页码范围
1798-1806
出版商
Springer US
简介
Purpose
Statins and certain calcium channel blockers may improve nitric oxide (NO) release and endothelial function through various mechanisms, but their combined effects are not well understood.
Methods
The separate versus combined effects of amlodipine (AML) and atorvastatin (AT) on NO and peroxynitrite (ONOO−) were measured in human umbilical vein endothelial cells (HUVEC) in the presence and absence of low-density lipoprotein (LDL) using electrochemical nanosensors.
Results
The combination of AML (5 μmol/l) and AT (3-6 μmol/l) directly stimulated NO release that was about twofold greater than the sum of their separate effects (p < 0.05). This synergistic activity is attributed to enhanced endothelial NO synthase (eNOS) function and decreased cytotoxic ONOO−. LDL (100 mg/dl) caused a …
引用总数
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RP Mason, R Kubant, G Heeba, RF Jacob, CA Day… - Pharmaceutical research, 2008