作者
Shiyong Wu, Lei Wang, Adam M Jacoby, Krystian Jasinski, Ruslan Kubant, Tadeusz Malinski
发表日期
2010/3
期刊
Photochemistry and photobiology
卷号
86
期号
2
页码范围
389-396
出版商
Blackwell Publishing Ltd
简介
Elevation of nitric oxide (NO˙) can either promote or inhibit ultraviolet B light (UVB)‐induced apoptosis. In this study, we determined real‐time concentration of NO˙ and peroxynitrite (ONOO−) and their role in regulation of membrane integrity and apoptosis. Nanosensors (diameter 300–500 nm) were used for direct in situ simultaneous measurements of NO˙ and ONOO− generated by UVB in cultured keratinocytes and mice epidermis. An exposure of keratinocytes to UVB immediately generated ONOO− at maximal concentration of 190 nm followed by NO˙ release with a maximal concentration of 91 nm. The kinetics of UVB‐induced NO˙/ONOO− was in contrast to cNOS agonist stimulated NO˙/ONOO− from keratinocytes. After stimulating cNOS by calcium ionophore (CaI), NO˙ release from keratinocytes was followed by ONOO− production. The [NO˙] to [ONOO−] ratio generated by UVB decreased below 0.5 …
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S Wu, L Wang, AM Jacoby, K Jasinski, R Kubant… - Photochemistry and photobiology, 2010