作者
Cristina A Metildi, Sharmeela Kaushal, Claudia Lee, Chanae R Hardamon, Cynthia S Snyder, George A Luiken, Mark A Talamini, Robert M Hoffman, Michael Bouvet
发表日期
2012/6/1
期刊
Journal of the American College of Surgeons
卷号
214
期号
6
页码范围
997-1007e2
出版商
LWW
简介
Background
The aim of this study was to improve fluorescence laparoscopy of pancreatic cancer in an orthotopic mouse model with the use of a light-emitting diode (LED) light source and optimal fluorophore combinations.
Study Design
Human pancreatic cancer models were established with fluorescent FG-RFP, MiaPaca2-GFP, BxPC-3-RFP, and BxPC-3 cancer cells implanted in 6-week-old female athymic mice. Two weeks postimplantation, diagnostic laparoscopy was performed with a Stryker L9000 LED light source or a Stryker X8000 xenon light source 24 hours after tail-vein injection of CEA antibodies conjugated with Alexa 488 or Alexa 555. Cancer lesions were detected and localized under each light mode. Intravital images were also obtained with the OV-100 Olympus and Maestro CRI Small Animal Imaging Systems, serving as a positive control. Tumors were collected for histologic analysis.
Results …
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CA Metildi, S Kaushal, C Lee, CR Hardamon… - Journal of the American College of Surgeons, 2012