作者
Elahe Motevaseli, Rosa Azam, Seyed Mohammad Akrami, Mohammadali Mazlomy, Mojtaba Saffari, Mohammad Hossein Modarressi, Maryam Daneshvar, Soudeh Ghafouri-Fard
发表日期
2016
期刊
Cell Journal (Yakhteh)
卷号
17
期号
4
页码范围
601
出版商
Royan Institute
简介
Objective
The aim of this study was to clarify the mechanism by which lactobacilli exert their cytotoxic effects on cervical cancer cells. In addition, we aimed to evalu-ate the effect of lactobacilli on the expression of human papilloma virus (HPV) onco-genes.
Materials and Methods
In this experimental study, using quantitative real-time polymer-ase chain reaction (PCR), we analyzed the expression of CASP3 and three autophagy genes [ATG14, BECN1 and alpha 2 catalytic subunit of AMPK (PRKAA2)] along with HPV18 E6 and E7 genes in HeLa cells before and after treatment with Lactobacillus crispatus and Lactobacillus rhamnosus culture supernatants.
Results
The expression of CASP3 and autophagy genes in HeLa cells was de-creased after treatment with lactobacilli culture supernatants. However, this de-crease was not significant for PRKAA2 when compared with controls. In addition, expression of HPV E6 was …
学术搜索中的文章
E Motevaseli, R Azam, SM Akrami, M Mazlomy… - Cell Journal (Yakhteh), 2016