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This review illustrated the role of metal-binding proteins (MBPs) and microbial interaction in assisting the phytoremediation of industrial wastewater polluted with heavy metals. MBPs are used to increase the accumulation and tolerance of metals by microorganisms via binding protein synthesis. Microbes have various protection mechanisms to heavy metals stress like compartmentalization, exclusion, complexity rendering, and the synthesis of binding proteins. MBPs include phytochelatins, metallothioneins, Cd-binding peptides (CdBPs), cysteines (gcgcpcgcg) (CP), and histidines (ghhphg)₂ (HP). In comparison with other physico-chemical methods, phytoremediation is an eco-friendly and safe method for the society. The present review concentrated on the efficiency of phytoremediation strategies for the use of MBPs and microbe-assisted approaches.