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The application of algal–bacterial symbiotic interaction as a low-cost and environment-friendly alternative for wastewater treatment was investigated in this research. In this context, a photobioreactor working under sequencing batch mode (SBR) was used. It consists of three basins of 200 L which were fed with pre-treated domestic wastewater. Following the adaptation period, and according to the obtained results, a synergistic interaction between algal and bacterial cultures was developed, which ultimately lead to enhance the removal of different pollutants. During daylight cycles, the dissolved oxygen concentration was around 6 mg/L, which promoted aerobic microbial activities to degrade organic pollutants such that more than 60% of the COD was removed. Additionally, more than 40% and 90% of the nitrogen (NH 4+-N) and phosphorus were removed, respectively. Based on multivariate data analysis, it was …