作者
Brent W Sutherland, Jill Kucab, Joyce Wu, Cathy Lee, Maggie CU Cheang, Erika Yorida, Dmitry Turbin, Shoukat Dedhar, Colleen Nelson, Michael Pollak, H Leighton Grimes, Kathy Miller, Sunil Badve, David Huntsman, C Blake-Gilks, Min Chen, Catherine J Pallen, Sandra E Dunn
发表日期
2005/6
期刊
Oncogene
卷号
24
期号
26
页码范围
4281-4292
出版商
Nature Publishing Group
简介
Akt/PKB is a serine/threonine kinase that promotes tumor cell growth by phosphorylating transcription factors and cell cycle proteins. There is particular interest in finding tumor-specific substrates for Akt to understand how this protein functions in cancer and to provide new avenues for therapeutic targeting. Our laboratory sought to identify novel Akt substrates that are expressed in breast cancer. In this study, we determined that activated Akt is positively correlated with the protein expression of the transcription/translation factor Y-box binding protein-1 (YB-1) in primary breast cancer by screening tumor tissue microarrays. We therefore questioned whether Akt and YB-1 might be functionally linked. Herein, we illustrate that activated Akt binds to and phosphorylates the YB-1 cold shock domain at Ser102. We then addressed the functional significance of disrupting Ser102 by mutating it to Ala102. Following the stable …
引用总数
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BW Sutherland, J Kucab, J Wu, C Lee, MCU Cheang… - Oncogene, 2005