作者
Sandra E Dunn, Michael Ehrlich, Nicholas JH Sharp, Kris Reiss, Gregory Solomon, Rebecca Hawkins, Renato Baserga, J Carl Barrett
发表日期
1998/8/1
期刊
Cancer research
卷号
58
期号
15
页码范围
3353-3361
出版商
The American Association for Cancer Research
简介
The 5-year survival rate for women with metastatic breast cancer is only 25–30%; thus, the need to improve treatment is apparent. Overexpression of insulin-like growth factor-I receptor (IGF-IR) correlates with poor prognosis and local recurrence. In this study, we addressed whether functional impairment of IGF-IR affects adhesion, invasion, and metastasis of breast cancer. Impairment of IGF-IR function was achieved by transfecting a dominant negative form of the receptor, termed 486stop, into MDA-MB-435 metastatic breast cancer cells. The protein product of 486stop is secreted extracellularly, resulting in a bystander effect. Cellular adhesion to laminin and collagen was inhibited 94 and 88%, respectively. Furthermore, 486stop inhibited insulin-like growth factor-I-stimulated invasion through collagen IV by 75%. The dominant negative receptor was secreted, as evidenced by the observation that MDA-MB-435 and …
引用总数
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SE Dunn, M Ehrlich, NJH Sharp, K Reiss, G Solomon… - Cancer research, 1998