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In the last one-decade numbers of review and research, articles have been published on niosomes. This shows the interest of researchers in niosomes because of the advantages offered by them over other vesicular carrier systems. Niosomes formation occurs when non-ionic surfactant vesicles assemble themselves. There are several factors like the type of non-ionic surfactant used, method of preparation, the temperature of hydration, etc. which affect the niosome formation. In this review article, we have made an attempt to incorporate all the basic details of niosomes like various methods of preparation, different types of niosomes, factors affecting their formation, characterization of niosome, their applications, routes of administration as well as the advancements taken place in the field of niosomal research with a literature review of research done in the last decade.