作者
Loris De Cecco, Luigi Marchionni, Manuela Gariboldi, James F Reid, M Stefania Lagonigro, Stefano Caramuta, Cristina Ferrario, Erica Bussani, Delia Mezzanzanica, Fabio Turatti, Domenico Delia, Maria G Daidone, Maria Oggionni, Norma Bertuletti, Antonino Ditto, Francesco Raspagliesi, Silvana Pilotti, Marco A Pierotti, Silvana Canevari, Claudio Schneider
发表日期
2004/10
期刊
Oncogene
卷号
23
期号
49
页码范围
8171-8183
出版商
Nature Publishing Group
简介
Epithelial ovarian cancer (EOC) is the gynecological disease with the highest death rate. We applied an automatic class discovery procedure based on gene expression profiling to stages III–IV tumors to search for molecular signatures associated with the biological properties and progression of EOC. Using a complementary DNA microarray containing 4451 cancer-related, sequence-verified features, we identified a subset of EOC characterized by the expression of numerous genes related to the extracellular matrix (ECM) and its remodeling, along with elements of the fibroblast growth factor 2 (FGF2) signaling pathway. A total of 10 genes were validated by quantitative real-time polymerase chain reaction, and coexpression of FGF2 and fibroblast growth factor receptor 4 in tumor cells was revealed by immunohistochemistry, confirming the reliability of gene expression by cDNA microarray. Since the functional …
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LD Cecco, L Marchionni, M Gariboldi, JF Reid… - Oncogene, 2004