作者
Byron Calgua, Tulio Fumian, Marta Rusinol, Jesus Rodriguez-Manzano, Viviana A Mbayed, Silvia Bofill-Mas, Marize Miagostovich, Rosina Girones
发表日期
2013/5/15
期刊
Water research
卷号
47
期号
8
页码范围
2797-2810
出版商
Pergamon
简介
Molecular techniques and virus concentration methods have shown that previously unknown viruses are shed by humans and animals, and may be transmitted by sewage-contaminated water. In the present study, 10-L river-water samples from urban areas in Barcelona, Spain and Rio Janeiro, Brazil, have been analyzed to evaluate the viral dissemination of human viruses, validating also a low-cost concentration method for virus quantification in fresh water. Three viral groups were analyzed: (i) recently reported viruses, klassevirus (KV), asfarvirus-like virus (ASFLV), and the polyomaviruses Merkel cell (MCPyV), KI (KIPyV) and WU (WUPyV); (ii) the gastroenteritis agents noroviruses (NoV) and rotaviruses (RV); and (iii) the human fecal viral indicators in water, human adenoviruses (HAdV) and JC polyomaviruses (JCPyV). Virus detection was based on nested and quantitative PCR assays. For KV and ASFLV …
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B Calgua, T Fumian, M Rusinol, J Rodriguez-Manzano… - Water research, 2013