作者
Amy Midori James, Dawei Ma, Robin Mellway, Andreas Gesell, Kazuko Yoshida, Vincent Walker, Lan Tran, Don Stewart, Michael Reichelt, Jussi Suvanto, Juha-Pekka Salminen, Jonathan Gershenzon, Armand Séguin, C Peter Constabel
发表日期
2017/5/1
期刊
Plant Physiology
卷号
174
期号
1
页码范围
154-171
出版商
American Society of Plant Biologists
简介
The accumulation of proanthocyanidins is regulated by a complex of transcription factors composed of R2R3 MYB, basic helix-loop-helix, and WD40 proteins that activate the promoters of biosynthetic genes. In poplar (genus Populus), MYB134 is known to regulate proanthocyanidin biosynthesis by activating key flavonoid genes. Here, we characterize a second MYB regulator of proanthocyanidins, MYB115. Transgenic poplar overexpressing MYB115 showed a high-proanthocyanidin phenotype and reduced salicinoid accumulation, similar to the effects of MYB134 overexpression. Transcriptomic analysis of MYB115- and MYB134-overexpressing poplar plants identified a set of common up-regulated genes encoding proanthocyanidin biosynthetic enzymes and several novel uncharacterized MYB transcriptional repressors. Transient expression experiments demonstrated the capacity of both MYB134 and …
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AM James, D Ma, R Mellway, A Gesell, K Yoshida… - Plant Physiology, 2017