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In this study we have attempted to identify and quantify phytosterols from four samples of Glycyrrhiza glabra harvested from four different areas (Bilekh, Rakka, Alpo-Azaz, Rass-Aenn) of Syria. Beta-sitosterol and stigmasterol are predominant in all samples. The highest amount of beta-sitosterol and campesterol was found in Alpo-Azaz sample and the biggest quantity of stigmasterol and ergosterol was found in Bilekh sample. This investigation offers an approach to rapidly characterization of phytosterols in herbal products.