作者
Nisar Ahmad, Hina Fazal, Bilal Haider Abbasi, Muhammad Rashid, Tariq Mahmood, Nighat Fatima
发表日期
2010/7
期刊
Plant Cell, Tissue and Organ Culture (PCTOC)
卷号
102
页码范围
129-134
出版商
Springer Netherlands
简介
The organogenic potential and antioxidant potential (1, 1-diphenyl-2-picrylhydrazyl-scavenging activity) of the medicinal plant Piper nigrum L. (black pepper) were investigated. Callus induction and shoot regeneration were induced from leaf explants of potted plants cultured on MS medium supplemented with different plant growth regulators. The best callogenic response was observed on explants cultured for 30 days on MS medium supplemented with either 0.5 or 1.5 mg l−1 6-benzyladenine (BA) + 1.0 mg l−1 α-naphthaleneacetic acid. Subsequent transfer of the callogenic explants onto MS medium supplemented with 1.5 mg l−1 BA + 1.0 mg l−1 gibberellic acid (GA3) achieved 85% shoot organogenesis after 30 days of culture. The maximum number (7.2) of shoots/explant was recorded for explants cultured in MS medium supplemented with 1.0 mg l−1 BA. Following the transfer of shoots to an …
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N Ahmad, H Fazal, BH Abbasi, M Rashid, T Mahmood… - Plant Cell, Tissue and Organ Culture (PCTOC), 2010