作者
Mario Vetrano, Federica d’Alessandro, Maria Rosaria Torrisi, Andrea Ferretti, Maria Chiara Vulpiani, Vincenzo Visco
发表日期
2011/12
期刊
Knee surgery, sports traumatology, arthroscopy
卷号
19
页码范围
2159-2168
出版商
Springer-Verlag
简介
Purpose
The aim of this study was to investigate whether the effects of extracorporeal shock wave therapy (ESWT) could affect the behavior of primary cultured human tenocytes over a 12-day period.
Methods
In this controlled laboratory study, primary human tenocytes were established from semitendinosus tendons collected from 3 patients undergoing arthroscopic anterior cruciate ligament (ACL) reconstruction. Cell viability, overall cell morphology, cell proliferation, and collagen synthesis following ESWT have been evaluated.
Results
ESWT significantly interferes with the overall cell morphology, by impairing dedifferentiation of the cells. Furthermore, a shock wave-mediated growth-promoting effect was measured by the MTT (tetrazolium) colorimetric assay and by the proliferation marker Ki67. Lastly, a significant …
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M Vetrano, F d'Alessandro, MR Torrisi, A Ferretti… - Knee surgery, sports traumatology, arthroscopy, 2011