作者
Qin He, Hazel McLellan, Richard K Hughes, Petra C Boevink, Miles Armstrong, Yuan Lu, Mark J Banfield, Zhendong Tian, Paul RJ Birch
发表日期
2019/4
期刊
New Phytologist
卷号
222
期号
1
页码范围
438-454
简介
- The potato blight agent Phytophthora infestans secretes a range of RXLR effectors to promote disease. Recent evidence indicates that some effectors suppress early pattern‐triggered immunity (PTI) following perception of microbe‐associated molecular patterns (MAMPs). Phytophthora infestans effector PiSFI3/Pi06087/PexRD16 has been previously shown to suppress MAMP‐triggered pFRK1‐Luciferase reporter gene activity. How PiSFI3 suppresses immunity is unknown.
- We employed yeast‐two‐hybrid (Y2H) assays, co‐immunoprecipitation, transcriptional silencing by RNA interference and virus‐induced gene silencing (VIGS), and X‐ray crystallography for structure‐guided mutagenesis, to investigate the function of PiSFI3 in targeting a plant U‐box‐kinase protein (StUBK) to suppress immunity.
- We discovered that PiSFI3 is active in the host nucleus and interacts in yeast and in planta with StUBK. UBK is a …
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