查看文章

An Initiation-Promotion Assay in Rat Liver as a Potential Complement to the 2-Year Carcinogenesis Bioassay. DRAGAN, Y. P., RIZVI, T., XU, Y.-H., HULLY, J. R., BAWA, N., CAMPBELL, H. A., MARONPOT, R. R., AND PITOT, H. C. (1991). Fundam. Appl. Toxicol. 16, 525–547. Several pharmaceutical agents, manufacturing chemicals, and environmental contaminants were found to act primarily as promoting agents in an initiation-promotion paradigm. The phenotypic distribution of four enzyme markers-placental glutathione-S-transferase (PGST), γ-glutamyl trans-peptidase (GGT), canalicular ATPase (ATPase), and glucose-6-phosphatase (G6Pase)-was analyzed in altered hepatic foci (AHF) by quantitative stereology. The number and volume distribution of AHF were determined for each promoter tested. For phenobarbital and 2,3,7,8-tetrachloro-p-dioxin, PGST and GGT together scored 100% of the AHF; for l …