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Behavioral and geographic variation in animal communication has been well-studied in insects, frogs, birds, and mammals, but little is known about variation in fishes. We used underwater audio-video recordings of the behavior and associated sounds produced by the domino damselfish, Dascyllus albisella, at Johnston Atoll and Hawaii, which are separated by 1000 km, to study behavioral and geographic variation in communication sounds. Males produced pulsed sounds during the courtship behavior known as the signal jump, visiting by females (during pseudospawning), mating, aggression to heterospecifics and conspecifics, and nest preparation. Females made only aggressive sounds. The following features of the sounds were measured: number of pulses, pulse rate, pulse duration, inter-pulse interval, dominant frequency, and frequency envelope. The only difference between visiting and mating …