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A field study was conducted on two texturally different soils to determine the influences of biosolids application on selected soil chemical properties and carbon dioxide fluxes. Two sites, located in Manildra (clay loam) and Grenfell (sandy loam), in Australia, were treated at a single level of 70 Mg ha⁻¹ biosolids. Soil samples were analyzed for SOC fractions, including total organic carbon (TOC), labile, and non-labile carbon contents. The natural abundances of soil δ¹³C and δ¹⁵N were measured as isotopic tracers to fingerprint carbon derived from biosolids. An automated soil respirometer was used to measure in-situ diurnal CO₂ fluxes, soil moisture, and temperature. Application of biosolids increased the surface (0–15 cm) soil TOC by > 45% at both sites, which was attributed to the direct contribution from residual carbon in the biosolids and also from the increased biomass production. At both sites application of …