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Background and Aims:  Eutypa dieback is a major threat to the sustainability and productivity of the viticulture industry worldwide. The fungicide benomyl has been the most effective treatment to protect wounds against infection by ascospores of Eutypa lata and to control eutypa dieback. However, because of the withdrawal of benomyl from the market, there is a need to develop alternatives to protect pruning wounds.

Methods and Results:  Twenty‐five compounds were evaluated in laboratory experiments and field trials. A selection of the compounds effective at inhibiting germination and/or mycelial growth in the laboratory were further evaluated in the field. Of the 15 fungicides tested, carbendazim was the most effective in reducing colonisation of pruning wounds by E. lata in the field. Other fungicides, including fluazinam, pyrimethanil and pyraclostrobin, reduced colonisation of wounds by E. lata but require …