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Three independent studies were conducted in Australia and Spain to examine the effect of water and temperature stress on the development of Eutypa dieback in grapevines. In Adelaide, South Australia, 'Red Grenache' vines in pots were inoculated with Eutypa lata and subjected to various temperature and soil water regimes, then assessed for foliar symptoms, wood staining and colonization by the fungus. Vines subjected to a combination of heat or cold plus low or high soil moisture displayed more severe foliar symptoms, the most severe occurring on vines subjected to the hottest (30°C) and wettest (20–40% soil water content) conditions. Wood staining was not related to foliar symptom severity or to the temperature and moisture combination. Furthermore, there was no relationship between staining and mycelial growth, indicating that staining may not be an accurate reflection of the spread of E. lata. In Cabrils …