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The aim of this study was to evaluate the effects of tumour necrosis factor-α (TNF-α) on sperm motility, mitochondrial membrane potential (ΔΨ), phosphatidylserine (PS) externalization, sperm chromatin packaging quality, and DNA fragmentation. Motile spermatozoa, obtained from 10 normozoospermic men, were incubated with increasing concentrations of TNF-α and analyzed 1, 3, 6, and 24 h after incubation by flow cytometry. TNF-α decreased total motility 24 h after incubation at 10 ng/mL and progressive motility 3 h after incubation. Accordingly, TNF-α reduced sperm ΔΨ in a concentration- and time-dependent manner. TNF-α increased the percentage of spermatozoa with PS externalization from the concentration of 1 ng/mL 1 h after incubation. TNF-α produced sperm chromatin and DNA damage in a concentration- and time-dependent manner. In conclusion, these findings may explain the reduction of fertility …