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Methods:

Newborn C57Bl6 mice, CD73-/-, and ENT2-/-mice were exposed to 95% oxygen or room air starting between 12 and 48 hours after birth. After 14 days exposure, surviving mice were sacrificed for collection of bronchoalveolar lavage fluid (BALF) and lungs for biochemical and histologic analysis. BALF was analyzed for adenosine concentrations and markers of inflammation. Lung sections were processed for histologic examination.

Results:

Exposure of C57Bl6 mice to hyperoxia increased BALF cell counts and protein concentrations, disrupted alveolar development and increased BALF adenosine concentrations. C57Bl6 mice had 56% survival in hyperoxia at 14 days. CD73-/-mice in hyperoxia all died by day 11. ENT-/-mice in hyperoxia all died by day 6.

Conclusions:

Hyperoxia exposure causes lung injury and disrupted alveolar development in newborn mice that is associated with increased extracellular …