作者
Fabrizio De Benedetti, Nadia Rucci, Andrea Del Fattore, Barbara Peruzzi, Rita Paro, Maurizio Longo, Marina Vivarelli, Flaminia Muratori, Silvia Berni, Paola Ballanti, Serge Ferrari, Anna Teti
发表日期
2006/11
期刊
Arthritis & Rheumatism: Official Journal of the American College of Rheumatology
卷号
54
期号
11
页码范围
3551-3563
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
Objective
To identify the mediator responsible for the impact of chronic inflammation on skeletal development in children (bone loss, defective peak bone mass accrual, stunted growth), we evaluated the effects of chronic interleukin‐6 (IL‐6) overexpression on the skeletons of growing prepubertal mice.
Methods
We studied IL‐6–transgenic mice that had high circulating IL‐6 levels since birth. Trabecular and cortical bone structure were analyzed by microcomputed tomography. Epiphyseal ossification, growth plates, and calvariae were studied by histology/histomorphometry. Osteoclastogenesis, osteoblast function/differentiation, and the effects of IL‐6 on bone cells were studied in vitro. Osteoblast gene expression was evaluated by reverse transcriptase–polymerase chain reaction. The mineral apposition rate was evaluated dynamically in cortical bone by in vivo double fluorescence labeling.
Results
In …
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