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A novel halotolerant actinomycete, strain Chem15^T, was isolated from soil around Inche-Broun hypersaline wetland; its taxonomic position was determined based on a polyphasic approach. Strain Chem15^T was strictly aerobic and tolerated NaCl up to 12.5 %. The optimum temperature and pH for growth were 28–30 °C and pH 7.0–7.5, respectively. The cell wall of strain Chem15^T contained meso-diaminopimelic acid as diamino acid and galactose, arabinose and ribose as whole-cell sugars. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The cellular fatty acids profile consisted of C_16 : 0, iso-C_18 : 0, C_18 : 0 10-methyl and C_18 : 1ω9c, and the major respiratory quinone was MK-8(H_4cycl). The G+C content of the genomic DNA was 68.0 mol%. The novel strain constituted a distinct …