作者
Mihaela Georgescu, Irina Gheorghe, Carmen Curutiu, Veronica Lazar, Coralia Bleotu, Mariana-Carmen Chifiriuc
发表日期
2016/3
期刊
BMC infectious diseases
卷号
16
页码范围
3-9
出版商
BioMed Central
简介
Background
The purpose of this study was to evaluate the virulence profiles of Pseudomonas aeruginosa clinical strains recently isolated from patients hospitalized for chronic leg ulcers in the Dermatology Department of Central Military Emergency University Hospital “Carol Davila”, Bucharest, Romania.
Methods
The phenotypic screening evaluated eight soluble virulence factors (haemolysins, lecithinase, lipase, caseinase, gelatinase, amylase, DNase, aesculin hydrolysis), as well as adherence ability (Cravioto adapted method) and invasion capacity on HeLa cells (gentamicin protection assay). Seven virulence genes encoding for protease IV, 3 exoenzymes (exoS, exoT, exoU), two phospholipases plcH- haemolytic phospholipase C and plcN- non-haemolytic phospholipase C) and alginate were investigated by PCR.
Results …
学术搜索中的文章
Virulence and resistance features of Pseudomonas aeruginosa strains isolated from chronic leg ulcers
M Georgescu, I Gheorghe, C Curutiu, V Lazar, C Bleotu… - BMC infectious diseases, 2016