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Thai basil is a well-known medicinal plant that is rich in bioactive antioxidant compounds having a variety of health benefits, including cancer, diabetes, and cardiovascular disease prevention. Plant cell and tissue culture technologies can be systematically developed as a valuable, low-cost biomass supply for the production of high-value phytochemicals. The aim of this study is to create an effective protocol for producing “Thai basil” leaf derived callus cultures with a high yield of biomass and antioxidants as an alternative to growing plants using salicylic acid and various light regimes as elicitors. Salicylic acid (SA) and light significantly affects many metabolic processes coupled with biosynthesis of secondary metabolites in plants. Callus was exploited with various SA concentrations under 3 different light regimes. Indeed, the results of our research disclose that continuous light with moderate SA concentration (10 …