查看文章

The dissolution–precipitation process for calcium‐phosphate ceramics in contact with biological fluid was studied by incubating blocks of biphasic calcium phosphate composed of hydroxyapatite (HA) and β‐tricalcium phosphate (β‐TCP) in different solutions: ionic simulated body fluid (SBF) without protein or SBF that contained various proteins and macromolecules separately (fibronectin, vitronectin, albumin, and poly‐L‐glutamic acid). Transmission electron microscopy studies revealed that apatite‐precipitated microcrystals appeared around ceramic crystals as a result of secondary nucleation; microcrystals were in continuity with the lattice planes of the HA crystals but in a different direction from that of β‐TCP; the size of the precipitates was smaller when fibronectin, vitronectin, and poly‐(L‐glutamic acid) were present in SBF as compared to SBF without protein; and fibronectin and vitronectin initiated crystal …