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The interaction of thioflavin T (ThT) with serum albumins from four different mammalian species i.e. human, bovine, porcine and rabbit, has been investigated by circular dichroism (CD), fluorescence spectroscopy and ITC. The binding constant (K) for HSA was found to be 9.9×10⁴M⁻¹, 4.3×10⁴M⁻¹ for RSA, 1.07×10⁴M⁻¹ for PSA and 0.3×10⁴M⁻¹ for BSA and the number of binding sites (n) were 1.14, 1.06, 0.94 and 0.8, respectively, which is very significant. By using unfolding pathway of HSA in the presence of urea, domain II of HSA has been assigned to possess binding site of ThT. Its binding constant is comparable to many drugs that bind at domain II of HSA, like salicylate, warfarin, digitoxin, etc. Acting force between HSA and ThT is showing that both hydrophobic and electrostatic forces have contributed for the interaction. ΔG_binding, ΔH and ΔS were calculated to be −28.46kJmol⁻¹, −3.50kJmol⁻¹ and 81 …