作者
Neshatul Haque, N Prakash Prabhu
发表日期
2016/10/1
期刊
Biochimica et Biophysica Acta (BBA)-General Subjects
卷号
1860
期号
10
页码范围
2326-2334
出版商
Elsevier
简介
Background
Understanding the dynamics of enzymes in organic solvents has wider implications on their industrial applications. Pancreatic lipases, which show activity in their lid open-state, demonstrate enhanced activity in organic solvents at higher temperatures. However, the lid dynamics of pancreatic lipases in non-aqueous environment is yet to be clearly understood.
Methods
Dynamics of porcine pancreatic lipase (PPL) in open and closed conformations was followed in ethanol, toluene, and octanol using molecular simulation methods. In silico double mutant D250V and E254L of PPL (PPLmut-Cl) was created and its lid opening dynamics in water and in octanol was analyzed.
Results
PPL showed increase in solvent accessible surface area and decrease in packing density as the polarity of the surrounded solvent decreased. Breaking the interactions between D250-Y115, and D250-E254 in PPLmut-Cl …
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N Haque, NP Prabhu - Biochimica et Biophysica Acta (BBA)-General Subjects, 2016