作者
Dianjun Cao, Qian M Cao, Sakthivel Subramaniam, Danielle M Yugo, C Lynn Heffron, Adam J Rogers, Scott P Kenney, Debin Tian, Shannon R Matzinger, Christopher Overend, Nicholas Catanzaro, Tanya LeRoith, Heng Wang, Pablo Piñeyro, Nicole Lindstrom, Sherrie Clark-Deener, Lijuan Yuan, Xiang-Jin Meng
发表日期
2017/7/7
期刊
Proceedings of the National Academy of Sciences of the United States of America
卷号
114
期号
27
页码范围
6914
出版商
National Academy of Sciences
简介
Results
Successful Establishment of a Pig Model for Chronic HEV Infection. To mimic the immunosuppressive conditions in human solid-organ transplant recipients, pigs in the immunocompromised group were orally administered a drug mixture compounded with three immunosuppressive drugs (see Materials and Methods for details) routinely used to prevent rejection in human organ transplant recipients. The immunosuppressive drug regimen was given 1 wk before infection with a genotype 3 human HEV as well as during the course of HEV infection to induce an effective nonspecific immunosuppression in pigs similar to that seen in organ transplant recipients.
Fecal virus shedding was initially monitored by HEV-specific regular RT-PCR. Viral RNAs were detected in the fecal samples of both drug-treated (immunocompromised) and mock-treated (immunocompetent) HEV-infected pigs up to 8 wk postinfection (wpi …
学术搜索中的文章
D Cao, QM Cao, S Subramaniam, DM Yugo… - Proceedings of the National Academy of Sciences of …, 2017