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ABSTRACT he purpose of this study was to determine the effect of using a progesterone-releasing intravaginal device (PRID) for treatment using the Cosynch-72 protocol combined with short term progesterone, which causes the concentration of acute phase markers in heifers. he material for this study consisted of 200 Holstein heifers which were 14-16 months of age and weighed 360-380 kg. In this protocol, blood was taken from the animals 10 days prior to synchronization, on day 0 (the day the PRID was inserted), on day 5 (the day PRID was removed) and on day 8 (the day of artiicial insemination). he values of haptoglobin (Hp), ceruloplasmin (CP), albumin, Fe, total iron-binding capacity (TIBC) and transferrin saturation (TS) were measured colorimetrically from the serum specimens which were taken. It was determined that the concentration of albumin, CP, Fe, TIBC and TS were similar on day-10 and day 0 (P> 0.05) but different on the other days (5 and 8), and that this change was statistically signiicant (P= 0.000). Consequently, it was determined that short term PRID treatment for the aim of synchronization in heifers increased the values of Hp, CP and TIBC, but decreased the concentration of albumin, Fe and TS.