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The aim of this study was to investigate the effect of β-carotene in acute phase response (APR) in rabbits which were administered high doses of toxic diethylnitrosamine (DEN). Twenty-one New Zealand race rabbits at 5-7 months of age were divided into 3 groups each having 7 ones. Control group received single dose of 0.9% NaCl solution intraperitoneally (IP); Group 1 received single dose of 100 mg/kg DEN (IP); Group 2 received both 100 mg/kg DEN (IP) and 2 mg/kg/day β-carotene orally for 7 days. After applying administrations, blood samples were obtained on 1st, 4th, and 7th days. The levels of ceruloplasmin was determined by the Colombo and Richterich technique; serum amyloid A (SAA) and haptoglobin using ELISA; iron (Fe) and unsaturated iron binding capacity (UIBC), AST and ALT using a commercial colorimetric kit; total iron binding capacity (TIBC) and transferrin saturation (TS) were determined using a formula based on Fe and UIBC. We determined that the levels of haptoglobin and SAA on 1st, 4th, and 7th days (P< 0.01) and the level of ceruloplasmin on 1st and 4th days significantly increased (P< 0.01) relative to the controls. On the other hand, the levels of TS decreased on 4th and 7th days relative to control (P< 0.05). These results suggest that β-carotene has a protective role on APR resulted from the toxic effect of DEN.