作者
Guy Polturak, Dario Breitel, Noam Grossman, Alejandro Sarrion‐Perdigones, Efrat Weithorn, Margarita Pliner, Diego Orzaez, Antonio Granell, Ilana Rogachev, Asaph Aharoni
发表日期
2016/4
期刊
New Phytologist
卷号
210
期号
1
页码范围
269-283
简介
  • Betalains are tyrosine‐derived red‐violet and yellow pigments, found in plants only of the Caryophyllales order. Although much progress has been made in recent years in the understanding of the betalain biosynthetic process, many questions remain open with regards to several of the proposed steps in the pathway. Most conspicuous by its absence is the characterization of the first committed step in the pathway, namely the 3‐hydroxylation of tyrosine to form l‐3,4‐dihydroxyphenylalanine (l‐DOPA).
  • We used transcriptome analysis of the betalain‐producing plants red beet (Beta vulgaris) and four o'clocks (Mirabilis jalapa) to identify a novel, betalain‐related cytochrome P450‐type gene, CYP76AD6, and carried out gene silencing and recombinant expression assays in Nicotiana benthamiana and yeast cells to examine its functionality.
  • l‐DOPA formation in red beet was found to be redundantly catalyzed by …
引用总数
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