作者
Felio J Bello, Jaime A Rodríguez, Jesús Escovar, Víctor A Olano, Alberto Morales, Martha González, Gloria Rey
发表日期
2001
期刊
Memórias do Instituto Oswaldo Cruz
卷号
96
页码范围
865-873
出版商
Instituto Oswaldo Cruz, Ministério da Saúde
简介
A new cell line, PC-0199-BR, was established from embryonated eggs of the mosquito Psorophora confinnis. To date (September 2000) it has had 62 continuous passages. This is the first report of a cell line of mosquitoes belonging to the genus Psorophora. Cell growth initially was achieved in the MM/VP12 medium, supplemented with 20% fetal bovine serum; however, the subcultures were later adapted to Grace's medium with 10% fetal bovine serum. Cell morphology in the primary cultures was heterogeneous; but later in the established cell line, the predominant cell type was epithelioid. Cultured cells were predominantly diploid (2n=6); however, chromosome abnormalities were observed in a small proportion of the cells in later passages. C and G band patterns were also determined in the karyotype. The cell line isozyme profiles coincided with pupae and adult samples of the species taken from the same colony. A preliminary arbovirus susceptibility study for the cell line was undertaken. No evidence was observed of contamination of the cell line with bacteria, fungi or mycoplasma.
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FJ Bello, JA Rodríguez, J Escovar, VA Olano… - Memórias do Instituto Oswaldo Cruz, 2001