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The purpose of experimental study was to develop a simulation software related “Intravenous Therapy and Drug Administration” and determine the effects of simulation software on learning of psychomotor skills and anxiety level in nursing education. Study sample included 69 students who met inclusion criteria of study at İstanbul University Florence Nightingale Nursing School. Data were collected three times as to before practice, after practice and before practice on humans by using an “Information Form”, “Intravenous Therapy and Drug Administration Skill Checklist”, “State-Trait Anxiety Inventory (STAI)”, “Intravenous Therapy and Drug Administration Simulation Software” and “Advanced Injection Arm”. Results present that simulation software is an effective method for learning and improving psychomotor skills and decreasing anxiety in nursing education. Generalizing simulation software in nursing education …