作者
Yui Shinojima, Tadashi Terui, Hiroyuki Hara, Makoto Kimura, Jun Igarashi, Xiaofei Wang, Hiroyuki Kawashima, Yujin Kobayashi, Satomi Muroi, Satoshi Hayakawa, Mariko Esumi, Kyoko Fujiwara, Srimoyee Ghosh, Tatsuo Yamamoto, William Held, Hiroki Nagase
发表日期
2010/8/1
期刊
Journal of dermatological science
卷号
59
期号
2
页码范围
98-106
出版商
Elsevier
简介
BACKGROUND
Epigenetic changes such as aberrant DNA methylation and histone modification have been shown to play an important role in the tumorigenesis of malignant melanoma.
OBJECTIVE
To identify novel tumor-specific differentially methylated regions (DMRs) in human malignant melanoma.
METHODS
The aberrant methylation at 14 candidate human genomic regions identified through a mouse model study with quantitative DNA methylation analysis using the Sequenom MassARRAY system was performed.
RESULTS
The CpG island Exon 1 region of the Zygote arrest 1 (ZAR1) gene, which is responsible for oocyte-to-embryo transition, showed frequent aberrant methylation of 28 out of 30 (93%) melanoma surgical specimens, 16 of 17 (94%) melanoma cell lines, 0% of 4 normal human epidermal melanocyte (NHEM) cell lines, 0% of 10 melanocytic nevi and 100% of 51 various cancer cell lines …
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Y Shinojima, T Terui, H Hara, M Kimura, J Igarashi… - Journal of dermatological science, 2010