作者
C Dominique Toran-Allerand, Rajesh C Miranda, WD Bentham, Farida Sohrabji, Theodore J Brown, Richard B Hochberg, Neil J MacLusky
发表日期
1992/5/15
期刊
Proceedings of the National Academy of Sciences
卷号
89
期号
10
页码范围
4668-4672
简介
The rodent and primate basal forebrain is a target of a family of endogenous peptide signaling molecules, the neurotrophins--nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3--and of the gonadal steroid hormone estrogen, both of which have been implicated in cholinergic function. To investigate whether or not these ligands may act on the same neurons in the developing and adult rodent basal forebrain, we combined autoradiography with 125I-labeled estrogen and either nonisotopic in situ hybridization histochemistry or immunohistochemistry. We now report colocalization of intranuclear estrogen binding sites with the mRNA and immunoreactive protein for the low-affinity nerve growth factor receptor, which binds all three neurotrophins, and for the cholinergic marker enzyme choline acetyltransferase (acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6). Colocalization of estrogen and low …
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CD Toran-Allerand, RC Miranda, WD Bentham… - Proceedings of the National Academy of Sciences, 1992