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The vector-borne diseases caused by mosquitoes are one of the major health problems around the world especially in tropical and sub-tropical countries. The present study was carried out to evaluate the larvicidal potential of petroleum ether, chloroform, ethyl acetate and methanol extracts of root and leaves of Senecio laetus Edgew. against the early fourth-instar larvae of the malarial vector, Anopheles stephensi; dengue vector, Aedes aegypti and the filarial vector, Culex quinquefasciatus. The root and leaf extracts of S. laetus at 200, 100, 50, 25 and 12.5 ppm caused a significant mortality of the tested mosquito species and root extracts showed the highest larvicidal potential than the leaf extracts. After 12 and 24 h of exposure period, the highest larvicidal activities were observed in the methanol extract of root with LC₅₀ = 24.68 and 16.70 ppm and LC₉₀ = 200.42 and 109.19 ppm for An. stephensi; LC₅₀ = 44.25 …