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This study examined the effects of Reactive red 239 on total soluble protein, peroxidase activity and malondialdehyde (MDA) content of corns. The corns were exposed to different concentrations of 4000, 5200, 6760, 8788 and 11424 mg/L Reactive Red 239. In comparison to the control group, there was an increase in the total soluble protein content of plant groups exposed to low-concentration dye application. On the contrary, total soluble protein content decreased as the concentration of the dye increased. In the analyzed plants, different effects were observed on peroxidase activity. When compared with the control group, a significant increase was identified in the plants which were treated with Reactive Red 239 excluding the group of 11424 mg/L application. Besides, the effects of Reactive Red 239 on MDA content were analyzed. MDA content of Reactive Red 239 treated plants was found to be lower in all application groups excluding 439 mg/L application group. Our findings indicated that Reactive Red 239 caused significant changes in total protein, peroxidase activity and MDA content.