作者
Nelson P Barrera, Shoshanna C Isaacson, Min Zhou, Vassiliy N Bavro, Alex Welch, Theresia A Schaedler, Markus A Seeger, Ricardo Núñez Miguel, Vladimir M Korkhov, Hendrik W Van Veen, Henrietta Venter, Adrian R Walmsley, Christopher G Tate, Carol V Robinson
发表日期
2009/8
期刊
Nature methods
卷号
6
期号
8
页码范围
585-587
出版商
Nature Publishing Group US
简介
We describe a general mass spectrometry approach to determine subunit stoichiometry and lipid binding in intact membrane protein complexes. By exploring conditions for preserving interactions during transmission into the gas phase and for optimally stripping away detergent, by subjecting the complex to multiple collisions, we released the intact complex largely devoid of detergent. This enabled us to characterize both subunit stoichiometry and lipid binding in 4 membrane protein complexes.
引用总数
学术搜索中的文章
NP Barrera, SC Isaacson, M Zhou, VN Bavro, A Welch… - Nature methods, 2009