查看文章

Multigeneration tests are very useful for the assessment of long term toxicity of pollutants such as endocrine disruptor compounds. In this study, multigeneration reproduction tests adapted from the ISO standard 11267 were conducted with the Collembola Folsomia candida. Springtails were exposed to artificial soil contaminated with four insect growth regulators (methoprene, fenoxycarb, teflubenzuron, and precocene II) according to two different experimental set-ups. In the first set-up, the parental generation (F₀) of Collembola was exposed to a pollutant for 28 days. Juveniles from the F₁ generation were transferred to uncontaminated soil for another 28-day period to generate the F₂ generation. In the second set-up, the F₀ generation was exposed to a pollutant for 10 days before being transferred to uncontaminated soil to reproduce. After 18–28 days, juveniles from the F₁ were transferred to clean soil to generate …