作者
Alexander W Lohman, Janelle L Weaver, Marie Billaud, Joanna K Sandilos, Rachael Griffiths, Adam C Straub, Silvia Penuela, Norbert Leitinger, Dale W Laird, Douglas A Bayliss, Brant E Isakson
发表日期
2012/11/16
期刊
Journal of Biological Chemistry
卷号
287
期号
47
页码范围
39602-39612
出版商
Elsevier
简介
S-Nitrosylation is a post-translational modification on cysteine(s) that can regulate protein function, and pannexin 1 (Panx1) channels are present in the vasculature, a tissue rich in nitric oxide (NO) species. Therefore, we investigated whether Panx1 can be S-nitrosylated and whether this modification can affect channel activity. Using the biotin switch assay, we found that application of the NO donor S-nitrosoglutathione (GSNO) or diethylammonium (Z)-1–1(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA NONOate) to human embryonic kidney (HEK) 293T cells expressing wild type (WT) Panx1 and mouse aortic endothelial cells induced Panx1 S-nitrosylation. Functionally, GSNO and DEA NONOate attenuated Panx1 currents; consistent with a role for S-nitrosylation, current inhibition was reversed by the reducing agent dithiothreitol and unaffected by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, a blocker of …
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AW Lohman, JL Weaver, M Billaud, JK Sandilos… - Journal of Biological Chemistry, 2012