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Banana allergy has been associated with the latex–fruit syndrome. Several IgE‐binding components, the relevant ones being proteins of 30–37‐kDa, have been detected in banana fruit, but none of them have been isolated and characterized yet. Objective To purify and characterize the 30–37  kDa banana allergens.

Fifteen patients allergic to banana were selected on the grounds of a latex‐allergic population. Prick by prick tests to this fruit were performed. Total and specific IgE to banana were determined. Banana allergens were isolated by affinity chromatography, followed by cation‐exchange chromatography. Their characterization includes N‐terminal sequencing, enzymatic activity assays, immunodetection with sera from allergic patients and with antichitinase antibodies, and CAP and immunoblot inhibition tests. Skin prick tests with banana extracts and with the purified allergens …